Journal: Molecular Medicine Reports

Article Title: Establishment and evaluation of a simulated high-altitude hypoxic brain injury model in SD rats

doi: 10.3892/mmr.2019.9939

Figure Lengend Snippet: Study design of the experimental procedure. SD, Sprague-Dawley; MDA, malondialdehyde; LDH, lactate dehydrogenase; SOD, superoxide dismutase; T-GSH, total glutathione; ELISA, enzyme-linked immunosorbent assay; H&E, hematoxylin and eosin; IHC, immunohistochemistry; HIF-1α, hypoxia inducible factor-1α.

Article Snippet: The total extraction sample kit (cat. no. AR0101-30), BCA protein assay kit (cat. no. AR0146), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protein sample buffer 2X (denaturation; cat. no. AR0131), broad spectrum protease inhibitor (cat. no. AR1182-1), broad spectrum phosphatase inhibitor (cat. no. AR1183), terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) apoptosis detection kit I-POD (cat. no. MK1025) and primary antibodies against Bcl-2-associated X protein (Bax; cat. no. BA0315), apoptotic protease activating factor-1 (Apaf-1; cat. no. BA2373), hypoxia inducible factor (HIF)-1α (cat. no. PB0245) and cytochrome c (cyto-c; cat. no. A03529) were obtained from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay, Immunohistochemistry

Journal: Molecular Medicine Reports

Article Title: Establishment and evaluation of a simulated high-altitude hypoxic brain injury model in SD rats

doi: 10.3892/mmr.2019.9939

Figure Lengend Snippet: Immunohistochemistry for caspase-3 and HIF-1α protein expression and Nissl's and TUNEL staining in the CA1 region in the hippocampus following hypoxia. (A) Nissl-stained neurons in the CA1 region of the hippocampus of SD rats. (B) TUNEL-positive apoptotic cells in the CA1 region of the hippocampus of SD rats. (C) Caspase-3 expression examined using immunohistochemical analysis and quantified. (D) HIF-1α expression examined using immunohistochemical analysis and quantified. Scale bar, 50 µm. Original magnification, ×200. The values are presented as the mean ± standard deviation. **P<0.01 with comparisons shown by lines. HIF-1α, hypoxia inducible factor-1α; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; SD, Sprague-Dawley; IOD, integrated optical density.

Article Snippet: The total extraction sample kit (cat. no. AR0101-30), BCA protein assay kit (cat. no. AR0146), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protein sample buffer 2X (denaturation; cat. no. AR0131), broad spectrum protease inhibitor (cat. no. AR1182-1), broad spectrum phosphatase inhibitor (cat. no. AR1183), terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) apoptosis detection kit I-POD (cat. no. MK1025) and primary antibodies against Bcl-2-associated X protein (Bax; cat. no. BA0315), apoptotic protease activating factor-1 (Apaf-1; cat. no. BA2373), hypoxia inducible factor (HIF)-1α (cat. no. PB0245) and cytochrome c (cyto-c; cat. no. A03529) were obtained from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: Immunohistochemistry, Expressing, TUNEL Assay, Staining, Immunohistochemical staining, Standard Deviation

Journal: Molecular Medicine Reports

Article Title: Establishment and evaluation of a simulated high-altitude hypoxic brain injury model in SD rats

doi: 10.3892/mmr.2019.9939

Figure Lengend Snippet: Hypoxia-activated mitochondrial apoptosis pathway. Western blot analysis for (A) caspase-3 and cleaved caspase-3, (B) Apaf-1, HIF-1α, Bax and cyto-c protein level in each group, n=6. (C) Quantified western blot analysis results. The values are presented as mean ± standard deviation. **P<0.01 with comparisons shown by lines. Apaf-1, apoptotic protease activating factor-1; HIF-1α, hypoxia inducible factor-1α; Bax, Bcl-2-associated X protein; cyto-c, cytochrome c.

Article Snippet: The total extraction sample kit (cat. no. AR0101-30), BCA protein assay kit (cat. no. AR0146), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protein sample buffer 2X (denaturation; cat. no. AR0131), broad spectrum protease inhibitor (cat. no. AR1182-1), broad spectrum phosphatase inhibitor (cat. no. AR1183), terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) apoptosis detection kit I-POD (cat. no. MK1025) and primary antibodies against Bcl-2-associated X protein (Bax; cat. no. BA0315), apoptotic protease activating factor-1 (Apaf-1; cat. no. BA2373), hypoxia inducible factor (HIF)-1α (cat. no. PB0245) and cytochrome c (cyto-c; cat. no. A03529) were obtained from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: Western Blot, Standard Deviation

Journal: Molecular Medicine Reports

Article Title: Establishment and evaluation of a simulated high-altitude hypoxic brain injury model in SD rats

doi: 10.3892/mmr.2019.9939

Figure Lengend Snippet: Schematic view of HIF-1α and the intrinsic triggering pathway of the apoptotic signaling cascade. Hypoxia stimulates the HIF-1α and mitochondrial apoptotic signaling pathway and triggers the release of cyto-c from the mitochondrial matrix to the cytoplasm, thereby binding to the Apaf-1 protein, inducing its conformational change and activating it. Thus, the apoptosome consisting of cyto-c/Apaf-1/caspase-9 is yielded and is able to cleave or activate the executioner caspase-3. The activated caspase-3 complex then results in apoptosis. C9, caspase-9; C3, caspase-3; HIF-1α, hypoxia inducible factor-1α; cyto-c, cytochrome c; Apaf-1, apoptotic protease activating factor-1; CARD, caspase recruitment domain containing; Bak, Bcl2 antagonist/killer; Bax, Bcl-2-associated X protein; Bcl-2, Bcl2, apoptosis regulator; CBP, CREB binding protein; HIF-1β, hypoxia inducible factor-1β; HNF4, hepatocyte nuclear factor 4α.

Article Snippet: The total extraction sample kit (cat. no. AR0101-30), BCA protein assay kit (cat. no. AR0146), sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protein sample buffer 2X (denaturation; cat. no. AR0131), broad spectrum protease inhibitor (cat. no. AR1182-1), broad spectrum phosphatase inhibitor (cat. no. AR1183), terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) apoptosis detection kit I-POD (cat. no. MK1025) and primary antibodies against Bcl-2-associated X protein (Bax; cat. no. BA0315), apoptotic protease activating factor-1 (Apaf-1; cat. no. BA2373), hypoxia inducible factor (HIF)-1α (cat. no. PB0245) and cytochrome c (cyto-c; cat. no. A03529) were obtained from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: Binding Assay